anti-AAV2 Replicase mouse monoclonal, 226.7, supernatant

anti-AAV2 Replicase mouse monoclonal, 226.7, supernatant

Catalog Number:
A001003109PRO
Mfr. No.:
65172
Price:
$526
  • Size:
    5 mL
    Quantity:
    Add to Cart:
      • Overview
        • Recognizes AAV replicase proteins Rep 78, Rep 68, Rep 52 and Rep 40 in AAV-infected cells. In Immunoblotting prominant reaction with Rep 78 and Rep 68, only faint reaction with Rep 52 and Rep 40.

          Please contact us at for specific academic pricing.

          Background

          Mab 226.7 reacts with Rep proteins (Rep78, Rep68, Rep52 and Rep40) of human AAV2-infected cells. The antibody shows low affinity which is most useful in affinity chromatography experiments where mild conditions can be used for dissociation of the antigen.
          In WB it reacts mainly with Rep52 and Rep40, faint reaction with Rep78 and Rep68 (long incubation time and short washing steps are recommended).
          Alternatives for WB: Cat. Nos 61069 /65169 and 61071 /65171 and for IF: Cat. Nos. 61073/65173

          More Details

      • Properties
        • Host
          mouse
          Antibody Type
          monoclonal
          Isotype
          IgG1
          Reactivity
          virus (AAV2)
          Clone
          226.7
          Immunogen
          Recombinant AAV2 Rep 78 protein, N-terminally truncated by 171 aa
          Purification
          hybridoma cell culture supernatant
          Conjugate
          Unconjugated
          Formulation
          contains 0.09% sodium azide
          Storage
          short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles

          * Research use only

      • Applications
        • Application
          Affinity Chromatography; ICC/IF; IP; WB
        • Application Dilutions
          Affinity Chromatography assay dependent
          Immunocytochemistry (ICC)/ Immunofluorescence (IF) assay dependent
          Immunoprecipitation (IP) assay dependent
          Western Blot (WB) 1:2 - 1:50

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