anti-AAV2 Replicase mouse monoclonal, 303.9, lyophilized, purified

Overview
- Recognizes AAV replicase proteins Rep 78, Rep 68, Rep 52 and Rep 40 in human AAV-2-infected cells.
  
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  Background
  
  Mab 303.9 reacts with Rep proteins (Rep78, Rep68, Rep52 and Rep40) of human AAV2-infected cells.
  
  More Details
  
  AAV Antibodies
Properties
- Host
  
  mouse
  
  Antibody Type
  
  monoclonal
  
  Isotype
  
  IgG1
  
  Reactivity
  
  virus (AAV2)
  
  Clone
  
  303.9
  
  Immunogen
  
  Recombinant AAV2 Rep 78 protein, N-terminally truncated by 171 aa
  
  Purification
  
  protein A affinity chromatography
  
  Conjugate
  
  Unconjugated
  
  Formulation
  
  lyophilized; reconstitute in 1 ml dist. water (final solution contains 0.09 % sodium azide, 0.5% BSA in PBS buffer, pH 7.4)
  
  Storage
  
  short term at 2-8°C; long term storage in aliquots at -20°C; avoid freeze/ thaw cycles
  
  * Research use only

Applications

Application

WB

Application	Dilutions
Western Blot (WB)	1:50 – 1:100

Application	Species	Publication
WB	AAV2	Article URL
WB	AAV2	Article URL
WB ICC-IF	AAV2	Article URL
WB	AAV2	Article URL
WB	AAV2	Article URL

Reference
- Dutheil, N. et al. Adeno-Associated Virus Rep Represses the Human Integration Site Promoter by Two Pathways That Are Similar to Those Required for the Regulation of the Viral p5 Promoter. J. Virol. 88, 8227-8241 (2014).
  Mietzsch, M. et al. OneBac: Platform for Scalable and High-Titer Production of Adeno-Associated Virus Serotype 1-12 Vectors for Gene Therapy. Hum. Gene Ther. 25, 212-222 (2014).
  GonÃ§alves, M. A. F. V et al. Targeted Chromosomal Insertion of Large DNA into the Human Genome by a Fiber-Modified High-Capacity Adenovirus-Based Vector System. PLoS One 3, (2008).
  Grimm, D., Kay, M. A. & Kleinschmidt, J. A. Helper virus-free, optically controllable, and two-plasmid-based production of adeno-associated virus vectors of serotypes 1 to 6. Mol. Ther. 7, 839-850 (2003).
  Wistuba, A., Weger, S., Kern, A., Rgen, J. & Kleinschmidt, A. Intermediates of Adeno-Associated Virus Type 2 Assembly: Identification of Soluble Complexes Containing Rep and Cap Proteins. J. Virol. 69, 5311-5319 (1995).