Alpha 2,3 Sialidase

This product is a recombinant neuraminidase (exo-α-sialidase, EC #3.2.1.18, CAS #9001-67-6), cloned from Streptococcus pneumoniae and expressed in Escherichia coli with an N-terminal 8xHis tag. The 8xHis tag may be removed by digestion with FasTEVTM (Cat #GE0501), a TEV protease with enhanced stability and catalytic activity.

This product preferentially releases terminal α2,3-linked N-acetylneuraminic acid (Neu5Ac) from oligosaccharides, complex carbohydrates, and glycoproteins.

This product does not contain any detectable activities or proteases or other glycosidases.

Unit Definition: One unit is defined as the amount of α2,3 Sialidase required to catalyze the release of 1 nanomole of p-nitrophenol (pNP) from 2-O-(p-Nitrophenyl)-α-D-N-acetylneuraminic acid (pNP-Neu5Ac) in 1 min at 37°C in 100 μL 1X Reaction Buffer 4 (50 mM EPPS, 100 mM NaCl, pH 7.5).
Product reconstitution: Dissolve the lyophilized product in 100 μL of ultrapure water to make a 50,000 units/ml (Cat #GE0302) solution in 1X Reaction Buffer 4 (50 mM Tris-HCl, 100 mM NaCl, pH 7.5). Once reconstituted, store at 4°C for up to 5 days or -20°C for up to 6 months. Aliquoting is recommended to avoid repeated freeze-thaw cycles.

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