O-GlcNAcase Enzyme: Specific Glycoprotein Modification

This product is a recombinant O-GlcNAcase or OGA (glycosyl hydrolase family GH84; EC 3.2.1.169), cloned from Oceanicola granulosus and expressed in Escherichia coli. It releases O-linked β-N-acetylglucosamine (GlcNAc) from serine and threonine residues of glycoproteins and glycopeptides.

This product does not contain any detectable activities of proteases, exo-β-N-acetylglucosaminidase, or other glycosidases.

Unit Definition: One unit is defined as the amount of O-GlcNAcase required to catalyze the release of 1 nmole p-nitrophenol (pNP) from p-nitrophenyl-N-acetyl-β-D-glucosaminide (pNP-GlcNAc) per min at 37°C in 100 μL 1X Reaction Buffer (50 mM Tris, 100 mM NaCl, pH 7.5).

Product reconstitution: Dissolve the lyophilized product in 100 μL of ultrapure water to make a 10,000 units/mL (Cat #GE0401) in 1X Reaction Buffer 4. Once reconstituted, store the enzyme at 4°C for up to 5 days or at -20°C for up to 3 months. Aliquoting is recommended to avoid repeated freeze-thaw cycles.

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