FasTEV Protease

FasTEV Protease

Catalog Number:
E001516012LEC
Mfr. No.:
GE0501
Price:
$300
  • Size:
    1,000 Units
    Quantity:
    Add to Cart:
      • Overview
        • Tobacco Etch Virus (TEV) Protease (EC#3.4.22.44) specifically cleaves the peptide bond between a glutamine and a glycine or serine of the consensus sequence, ENLYFQ↓(G/S).

          FasTEVTM is an engineered version of the enzyme with multiple mutations that enhance its stability and catalytic activity. It is recombinantly expressed in Escherichia coli with an N-terminal 8xHis tag.

          This product does not contain any detectable activities of nonspecific proteases.

          Unit Definition: One unit is defined as the amount of FasTEVTM required to cleave 90% of 0.1 nanomole (5.4 μg) of a fusion protein substrate in 1 h at 30°C in 20 μL 1X Reaction Buffer 5 (50 mM Tris, 50 mM NaCl, 1 mM EDTA, pH 7.5).

          Product reconstitution: Dissolve the lyophilized product in 100 μL molecular grade water to make a 10,000 units/mL (GE0501) solution in 1X Reaction Buffer 5 (50 mM Tris, 50 mM NaCl, 1 mM EDTA, pH 7.5). Once reconstituted, FastTEVTM can be stored at 4°C for up to 2 weeks or -20°C for up to 3 months. Aliquoting is recommended to avoid repeated freeze-thaw cycles.

          Please contact us at for specific academic pricing.

      • Properties
        • * For Research Use Only

      • Applications
        • Application
          Glycan Processing

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