PNGase F-II: Advanced Glycoprotein Deglycosylation

This product is recombinant PNGase F-II, cloned from Elizabethkingia meningosepticum and expressed in Escherichia coli. It catalyzes the cleavage of asparagine-linked oligosaccharides, with or without core fucose, from glycoproteins and glycopeptides. Unlike PNGase F, which cannot cleave N-glycans with α1,3-linked core fucose, PNGase F-II can cleave N-glycans with α1,6- or α1,3-linked core fucose.

This product does not contain any detectable activities of proteases or other glycosidases.

Unit Definition: One unit is defined as the amount of PNGase F-II required to deglycosylate 1 nanomole (15 μg) of denatured RNase B or 0.1 nanomole (4.5 μg) of horse radish peroxidase (HRP) in 2 h at 37°C in 25 μL 1X Reaction Buffer (50 mM Bis-Tris, 100 mM NaCl, pH 7.0).

Product reconstitution: Dissolve the lyophilized product in 100 μL ultrapure water to make a 1,000 units/mL solution in Storage Buffer (20 mM Tris-HCl, 200 mM NaCl, pH 7.5). Once reconstituted, store at 4°C for up to 5 days or -20°C for up to 6 months. Aliquoting is recommended to avoid repeated freeze-thaw cycles.

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