Name: Acridine Orange hydrochloride
Price: 153.00 USD

- Overview
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    Background
    
    Acridine orange hydrochloride is a cell and organelle membrane permeable fluorescent dye that targets nucleic acids. Acridine orange can either be incorporated into double helical nucleic acids (detected by green fluorescence at 530 nm), or bind electrostatically to phosphate groups of single-stranded molecules (detected by red fluorescence at 640 nm), which renders it the ability to differently stain DNA and RNA or single-stranded DNA in situ. For unfixed cells, acridine orange staining can still be used as a simple, fast approach of obtaining information on cell cycle status and cell ploidy levels from DNA staining (green fluorescence), as well as cell transcriptional activity from RNA measurements (red fluorescence), in a flow cytofluorometric system. Thus, acridine orange may serve as a useful cytochemical stain for cell-cycle and apoptosis studies.
    
    1. McMaster GK, Carmichael GG. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proceedings of the National Academy of Sciences of the United States of America, 1977, 74(11): 4835-4838.
    2. Traganos F, Darzynkiewicz Z, Sharpless T, et al. Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorometric system. Journal of Histochemistry and Cytochemistry, 1977, 25(1): 46-56.
- Properties
  - Categories
    
    A cell and organelle membrane permeable fluorescent dye which targets nucleic acids
    
    Alternative Name
    
    N3,N3,N6,N6-tetramethylacridine-3,6-diamine hydrochloride
    
    CAS Number
    
    65-61-2
    
    Molecular Formula
    
    C17H19N3·HCl
    
    Molecular Weight
    
    301.81
    
    Appearance
    
    A solid
    
    Purity
    
    98.00%
    
    Solubility
    
    ≥30.3 mg/mL in H2O; ≥30.5 mg/mL in EtOH; ≥30.6 mg/mL in DMSO with gentle warming
    
    Storage
    
    Store at RT
    
    SMILES
    
    CN(C)C1=CC2=NC3=CC(N(C)C)=CC=C3C=C2C=C1.Cl
    
    * For Research Use Only