AAV-shRNA Selection Kits

The AAV-shRNA Selection Kit is designed for efficient selection of shRNAs. Each AAV-shRNA Selection kit includes 4 pre-designed shRNA constructs targeting your gene of interest (GOI), 1 scramble shRNA as control, and a GOI-mCherry fusion reporter plasmid. All plasmids are carried two AAV ITRs, after selection, the constructs can directly used to package AAV vectors.

Key Features
· Pre-validated shRNA library (4 target-specific + 1 scrambled control)
· Dual-reporter system for simultaneous transfection monitoring & knockdown quantification
· Flexible promoter options for both shRNA and reporter expression
· Seamless transition from screening to AAV production

Kit Components
· Pre-designed shRNA Plasmids
4 target-specific shRNA constructs (eg. pAAVtri-U6-shRNA-CMV-EGFP)
· 1 scrambled control shRNA
Reporter Plasmid (pAAVtri-CMV-GOI-mCherry)
GOI-mCherry fusion for real-time knockdown assessment
· Backbone Flexibility
Reporter expression: CMV or EF1α promoters
shRNA expression: U6 or H1 promoters
· Dual-Reporter System
EGFP: Visualize transfection efficiency
mCherry: Quantify gene silencing efficacy

Workflow:
· Co-transfection: Deliver shRNA plasmids + GOI-mCherry plasmid into cells (e.g., HEK293T).
· Screening: Analyze mCherry/EGFP ratios (flow cytometry or qPCR) to identify optimal shRNAs.
· AAV Packaging: Selected shRNAs can be packaged into AAV vectors (individual or pooled purification).

Please contact us at for specific academic pricing.