Name: 96 Well Plasmid Kit
Price: 308.00 USD

- Overview
  - The Presto™ 96 Well Plasmid Kit was designed for high-throughput purification of plasmid DNA from 1-5 ml of cultured bacterial cells per well using an efficient 96 well filter plate and 96 well binding plate system. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 20-35 μg for high-copy number plasmid or 3-10 μg for low-copy number plasmid from 4 ml of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 25 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.
    
    Please contact us at for specific academic pricing.
- Properties
  - Details
    
    Purify 96 wells of pure plasmid DNA in 25 minutes!
    Sample Volume: 1-5 ml of cultured bacterial cells per well
    Yield: 20-35 μg per well (high-copy plasmid), 3-10 μg per well (low-copy plasmid)
    Binding Capacity: 35 μg per well
    Plasmid Size Range: 1-20 kb
    96 Well plates for filtration and DNA binding
    Elution Volume: 60 μl from 100 μl elution buffer volume
    Centrifuge or vacuum manifold protocol
    
    Storage
    
    dry at room temperature (15-25°C)
    
    * For Research Use Only
- Applications
  - Application
    
    Plasmid DNA Purification
    
    Application Description
    
    Fluorescent and Radioactive Sequencing, Restriction Enzyme Digestion, Library Screening, Ligation, Transformation and PCR

Documents

PROTOCOL

96 Well Plasmid Kit

96 Well Plasmid Kit

Documents

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