Gel/PCR DNA Fragments Kit

The Gel/PCR DNA Fragments Extraction Kit is a Gel Extraction Kit and PCR Cleanup Kit in 1 convenient system. This Gel Extraction Kit will recover or concentrate 70 bp-20 kb DNA fragments from agarose gel, PCR, or other enzymatic reactions. For processing 10-50 kb DNA fragments, the Large DNA Fragments Extraction Kit is available. For processing 40-200 bp DNA fragments, the Small DNA Fragments Extraction Kit is also available. The Gel/PCR DNA Fragments Extraction Kit uses chaotropic salt to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the gel/pcr spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions such as Fluorescent and Radioactive Sequencing, PCR, Restriction Enzyme Digestion, DNA Labeling and Ligation, Microarray and Transfection.

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