96 Well Bacteria DNA Kit

96 Well Bacteria DNA Kit

Catalog Number:
NAPK388935GEN
Mfr. No.:
96GBB02; 96GBB04; 96GBB10
Price:
$521
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      • Overview
        • The Presto™ 96 Well gDNA Bacteria Kit is optimized for high-throughput genomic and viral DNA purification from Gram (-) negative and Gram (+) positive bacterial cells. Gram+ Buffer, when combined with Lysozyme, will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer. Proteinase K and chaotropic salt are used to further lyse cells and degrade protein, allowing DNA to easily bind to the glass fiber matrix of the binding plate. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. Phenol/chloroform extraction or alcohol precipitation is not required and the purified genomic DNA is ready for use in a variety of downstream applications.

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      • Properties
        • Details
          High-throughput bacteria DNA extraction
          High Yield: up to 5 μg of genomic DNA per well
          High Purity gDNA: A260/A280 >1.9
          DNA Size: 20-40 kb
          Samples: Gram (+) Positive and Gram (-) Negative bacterial cells (up to 5 x 10⁸)
          Convenient: Includes Gram+ Buffer for preparing Lysozyme solutions to speed up sample preparation when purifying DNA from Gram (+) positive bacteria
          96 Well Binding Plates: glass fiber membrane optimized for genomic DNA extraction (sterilised to remove bacteria contamination)
          Elution volume: 100-200 µl
          Storage
          dry at room temperature (15-25°C), Lysozyme is shipped at room temperature and should be stored at -20°C for extended periods

          * For Research Use Only

      • Applications
        • Application
          Genomic DNA Purification
          Application Description
          PCR, Restriction Endonuclease Digestions, Array, Southern Blots and various other enzymatic reactions

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