96 Well DNA Bacteria Advanced Kit

- Overview
  - The Presto™ 96 Well DNA Bacteria Advanced Kit is optimized for high-throughput genomic DNA purification from Gram (-) negative and Gram (+) positive bacterial cells. After directly adding bacteria samples into beadbeating microtubes pre-filled with zirconia/ceramic beads and lysis buffer, cells are efficiently lysed via bead beating instrument without enzymatic digestion. The cell lysate is then mixed with a binding buffer and the genomic DNA is bound by the membrane in the 96 well plate, followed by wash and elution. The entire procedure can be completed within 90 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.
    
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- Properties
  - Details
    
    96 well bead beating microtubes pre-filled with zirconia/ceramic beads
    Enzymatic digestion is not required!
    High-throughput bacteria DNA extraction
    High Yield: up to 5 μg of genomic DNA per well
    High Purity gDNA: A260/A280 >1.9
    DNA Size: 20-40 kb
    Samples: Gram (+) Positive and Gram (-) Negative bacterial cells (up to 5 x 10⁸)
    96 Well Binding Plates: glass fiber membrane optimized for genomic DNA extraction (sterilised to remove bacteria contamination)
    Elution volume: 60-100 µl
    
    Storage
    
    dry at room temperature (15-25°C)
    
    * For Research Use Only
- Applications
  - Application
    
    Genomic DNA Purification
    
    Application Description
    
    PCR, Restriction Endonuclease Digestions, Array, Southern Blots and various other enzymatic reactions

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PROTOCOL

96 Well DNA Bacteria Advanced Kit

96 Well DNA Bacteria Advanced Kit

Documents

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