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Coomassie-stained SDS-PAGE
Lane 1: Molecular weight markers
Lane 2: 5 µg purified Ubiquitin(1-75)
Lane 3: 5 µg purified 6xHis-Ubiquitin(1-75)

Coomassie-stained SDS-PAGE Lane 1: Molecular weight markers Lane 2: 5 µg purified Ubiquitin(1-75) Lane 3: 5 µg purified 6xHis-Ubiquitin(1-75)

6xHis-Ubiquitin(1-75)

6xHis-Ubiquitin(1-75)

Catalog Number:

UULP097414UBP

Mfr. No.:

E1310

Price:

$250

Size:

1 mg

Quantity:

Add to Cart:

- Overview
  - Ub75 lacks the amino acid glycine 76, which is required to form thioester bond with Ub activating enzyme E1. Therefore, Ub75 can not react with E1, E2 and E3 enzymes; and does not support ubiquitination. It can be used as a negative control in ubiquitination reactions; or to assay binding of Ub with Ub binding proteins or ubiquitination enzymes.
    
    Please contact us at for specific academic pricing.
- Properties
  - Protein Name
    
    6xHis-Ub(1-75)
    
    Source
    
    Bacterial recombinant
    
    Formulation
    
    20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
    
    Storage
    
    Store at -80°C; avoid multiple freeze-thaw cycles
    
    Concentration
    
    See tube label
    
    Molecular Weight
    
    8.5 kDa
    
    * Research Use Only

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