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6xHis-Ubiquitin

6xHis-Ubiquitin

Catalog Number:

UULP097416UBP

Mfr. No.:

E1300

Price:

$220

Size:

1 mg

Quantity:

Add to Cart:

- Overview
  - Ubiquitin (Ub) is a 76 amino acid protein widely expressed in the cytoplasmic and nucleus of cells. Ub is posttranslationally conjugated to proteins by the E1, E2, E3 protein ubiquitination cascade. Ub can be conjugated on proteins as monoUb or polyUb chains. Protein ubiquitination plays both proteolytic and nonproteolytic functions. Usually, polyubiquitinated proteins are targeted to the 26S proteasome for proteolysis. Typical concentration to support in vitro ubiquitination is 50-100 µM.
    
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- Properties
  - Protein Name
    
    6xHis-Ub
    
    Source
    
    Bacterial recombinant
    
    Formulation
    
    20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
    
    Storage
    
    Store at -80°C; avoid multiple freeze-thaw cycles
    
    Concentration
    
    See tube label
    
    Molecular Weight
    
    8.5 kDa
    
    * Research Use Only

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