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Overview
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6-CDCFDA is a fluorescent cell tracker that can enter cells by passive diffusion and covalently bind to intracellular proteins. It is a long-acting cell tracing dye. Once inside the cell, the non-fluorescent 6-CDCFDA is hydrolyzed by intracellular esterases to produce green fluorescence. These fluorescent products can only accumulate in cells with intact cell membranes, so dead cells with damaged membranes cannot be stained. 6-CDCFDA is not sensitive to pH changes and can be fixed with formaldehyde or glutaraldehyde.
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