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Overview
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DiI is a lipophilic fluorescent dye that can be used to stain cell membranes and other lipophilic biological structures. Once incorporated into the cell membrane, DiI diffuses throughout the membrane, and at optimal concentrations, it can stain the entire cell membrane. The fluorescence of DiI is very weak before it enters the cell membrane, but its fluorescence intensity significantly increases upon binding to the membrane. Upon excitation, DiI emits an orange-red fluorescence, characterized by a high quenching constant, moderate photon intensity, and a short excited-state lifetime. It can be detected using standard TRITC filter sets.
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- Properties
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Overview