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Overview
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DiD is a member of the lipophilic fluorescent dye family and can be used to stain cell membranes and other lipophilic biological structures. When DiD binds to the cell membrane, its fluorescence intensity is greatly enhanced. These dyes have a high quenching constant and a long excited-state lifetime. Once the cells are stained, the dyes diffuse across the entire cell membrane and can stain the entire cell membrane at the optimal concentration. DiD (far-red fluorescence) can be used for imaging and flow cytometry of live cells. DiD can be excited by a 633 nm He–Ne laser and has longer excitation and emission wavelengths than DiI (a common fluorescent cell dye), making it more valuable for cell and tissue staining.
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- Properties
- Applications
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Overview