Name: 5,5-Dithiobis(2-nitrobenzoic acid)
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- Overview
  - DTNB is known as Ellmans reagent. It is used for the colorimetric determination of thiol groups in biological samples.It is fairly soluble in water. Colorless DTNB is converted to yellow 5-Mercapto-2-nitrobenzoic acid in the presence of thiol compounds. For example, DTNB in combination with S-acetylthiocholine iodide as the substrate is widely used to assay choline esterase activity.
    
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- Properties
  - Categories
    
    Reagents
    
    Alternative Name
    
    Ellmans reagent;3-Carboxy-4-nitrophenyl disulfide;Bis(3-carboxy-4-nitrophenyl) disulfide;DTNB
    
    CAS Number
    
    69-78-3
    
    Molecular Formula
    
    C14H8N2O8S2
    
    Molecular Weight
    
    396.35 g/mol
    
    MDL Number
    
    MFCD00007140
    
    Note
    
    There is no hazardous surcharge associated with this product.
    There is no special packaging charge associated with this product.
    
    * For research use only. Not for human or veterinary use
- Reference
  - Riddles et al (1979). Ellman’s Reagent: 5,5’-Dithiobis(2-nitrobenzoic Acid) - a Re-examination. Analytical biochemistry. 94:75-81
    Ellman (1959). Tissue sulfhydryl groups. Archives of biochemistry and biophysics 82:70-77