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Overview
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5-Bromo-4-chloro-3-indolyl α-D-glucopyranoside (X-Gluc), is an indolyl carbohydrate and a Chromogenic substrate forα-D-Glucosidase that derives from an indoxyl. Upon enzymatic reaction, 5-Bromo-4-chloro-3-indolyl α-D-glucopyranoside forms indigo-blue precipitate which is used is used for the detection of α-Glucosidase positive organisms, e.g. Staphylococcus aureus and Bacillus species, and the isolation of Enterobacter sakazakii. It is the alpha-D-galactoside of indoxyl whereby the indole moiety issubstituted at positions 4 and 5 by chlorine and bromine, respectively.
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