33mer gliadin peptide

- Overview
  - 33mer gliadin peptide is a fusion protein consisting of the peptide sequence “LQLQPFPQPQLPYPQPQLPYPQPQLPYPQPQPF” and a carrier protein. It can be used as an antigen for the detection of antibodies specific for native alpha gliadin.
    
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    More Details
    
    Cereal Proteins
- Properties
  - Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    > 95% (SDS-PAGE and Coomassie staining)
    
    Formulation
    
    33mer gliadin peptide is lyophilized from a solution of ~50 mM NaH2PO4, pH 6.8.
    
    Storage
    
    Store at ≤ - 20°C. Store working aliquots at ≤ - 20°C. Avoid repeated freezing and thawing. Delivery is possible at ambient temperature.
    
    Note
    
    INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
    
    Molecular Weight
    
    27 kDa
    
    Class
    
    Recombinant gliadin Peptides
    
    Reconstitution
    
    Add the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. Further dilutions can be prepared in your buffer of choice. After reconstitution, the solution should be stored frozen in working aliquots.
- Applications
  - Application Description
    
    The recombinant antigen is meant for solid (ELISA and immunoblot) and fluid phase assays as well as Western Blotting.