DGPx1

- Overview
  - DGPx1 is a fusion protein of a deamidated gliadin peptide fused with a carrier protein to be used as antigen for the detection of antibodies specific for deamidated gliadin. The deamidated gliadin peptide is based on the 26-mer gamma gliadin (Dørum S. et al., J Proteome Res. 2009; 8:1748-55).
    
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    Cereal Proteins
- Properties
  - Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    > 95% (SDS-PAGE and Coomassie staining)
    
    Formulation
    
    DGPx1 is lyophilized from a solution of ~50 mM NaH2PO4, pH 6.8.
    
    Storage
    
    Store at -80°C. If storage at -80°C is not possible, storage at ≤ -20°C is recommended. While no formal stability dataare available at -20°C, according to our overall experience stability is still given. Store working aliquots preferably at -80°C (if not possible at ≤ -20°C, see comment above). Avoid repeated freezing and thawing. Delivery is possible at ambient temperature.
    
    Note
    
    Intended for research use only, not for use in human, therapeutic or diagnostic applications.
    
    Molecular Weight
    
    26 kDa
    
    Protein Name
    
    26mer γ-gliadin, deamidated; 26mer DGP; Deamidated Gliadin Peptide – fusion protein
    
    Class
    
    Recombinant gliadin Peptides
    
    Reconstitution
    
    Add at least the volume of H2O the protein is lyophilized from (see Certificate of Analysis) to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots.
- Applications
  - Application Description
    
    The recombinant antigen is meant for solid (ELISA and immuno blot) and fluid phase diagnostic assays. The protein is bound by human type IgA and IgG (auto) antibodies.

Datasheet

MSDS

DGPx1

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