2× Hieff™ HotStart PCR Genotyping Master Mix(With Dye)

2×Hieff™ HotStart PCR Genotyping Master Mix (With Dye) is a ready-to-use PCR pre-mixed solution containing Hieff™ HotStart Taq DNA Polymerase, dNTPs, and an optimized buffer system. It simplifies experimental procedures significantly by requiring only the addition of primers and template for amplification, enabling high-throughput operations and improving reproducibility of experimental results. Hieff™ HotStart Taq DNA Polymerase is a ligand-modified thermostable Taq DNA Polymerase whose activity is modulated with temperature changes. Its enzyme activity is completely blocked at room temperature and released upon heating at 95°C. Activation of Hieff™ HotStart DNA Polymerase requires only 2-3 minutes and is compatible with existing PCR programs. This product prevents non-specific amplification during sample preparation and temperature cycling stages, making it effective for genotyping experiments.

Features
• Convenient, ready-to-use mix
• Thermostable—half-life is more than 40 min at 95°C
• Generates PCR products with 3'-dA overhangs

Applications
• Mouse genotyping
• Hotstart PCR

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