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Overview
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Hifair™ Lyo-Ready I Multiplex One Step RT-qPCR Kit(UDG plus) is an optimized reagent for fast and sensitive amplification and quantitation of RNA targets with probe-based assays. In the process of the experiment, reverse transcription and quantitative PCR were carried out in the same tube, which simplified the experimental operation and reduced the risk of contamination.The unique Buffer and enzyme design ensures that it can be used in the preparation and product design of one-step RT-qPCR lyophilized reaction systems.
The kit mainly contains Hifair™ V Reverse Transcriptase, Hieff UniconTM HotStart Taq DNA Polymerase and optimized lyophilizable buffer,etc. In addition, the factors that can effectively inhibit the non-specific PCR amplification and improve the amplification efficiency of multiple qPCR reactions are added, which can ensure the amplification efficiency and carry out up to multiple amplification reaction.
This kit is fitted with lyoprotectant that can be used for lyophilization.
Features
• Broad Compatibility: Compatible with both direct-use and freeze-dried formats for long-term storage
• High Stability: Maintains excellent stability after lyophilization, even when stored at 50°C for up to 33 days
• Flexible Lyophilization Formats: Supports various formats including 8-tube strip in-situ lyophilization, vial-based lyophilization, and micro-core freeze-dried forms
• Rapid Workflow: Delivers results in as fast as 40 minutes with an optimized fast-cycling protocol
• Effective Contamination Control: Incorporates a UDG/dUTP system for efficient prevention and removal of carryover contaminationPlease contact us at for specific academic pricing.
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Overview