ZIP (SCRAMBLED)

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    IC50: By suppressing protein kinase Mξ (PKMξ), ZIP reverses late-phase LTP with an IC50 of 1 - 2.5 μM.Long-term potentiation (LTP), a persistent synaptic enhancement, is considered to be a substrate for memory. A typical LTP process includes two phases, induction and maintenance. PKMξ as an active form of protein kinase C (PKC) isozyme is necessary and potent for LTP maintenance. ZIP is applied as a novel and cell-permeable inhibitor for PKMξ and can therefore block LTP. [1]In vitro: In order to determine the specific phase of LTP affected by PKMξ, ZIP was added at different concentrations to the bath before cells incubation. Experiments with ZIP were compared with experiments without the peptide. This study showed that ZIP could selectively inhibit PKMξ-induced synaptic potentiation in hippocampal slices in vitro. [2]In vivo: ZIP was the first tool available to test the maintenance hypothesis in vitro. The effect of ZIP on late phase of LTP in vivo was also detected using the rat hippocampus. It was found that intra-hippocampal injection of ZIP with a dosage of 10 nmol in 1 ml saline rapidly reversed the late-phase LTP and led to persistent loss of 1-day-old spatial memory. [3]Clinical trial: So far, no clinical trial has been conducted.
    
    [1]Ling SF, Benardo LS, Serrano PA, Blace N, Kelly MT, Crary JF and Sacktor TC. Protein kinase Mξ is necessary and sufficient for LTP maintenance. Nat. Neurosci. 2002 Apr. 5(4): 2956.
    [2]Serrano P, Yao Y and Sacktor TC. Persistent phosphorylation by protein kinase maintains late-phase long-term potentiation. J. Neurosci. 2005 Feb. 25(8): 1979–84.
    [3]Pastalkova E, Serrano P, Pinkhasova D, Wallace E, Fenton AA, Sacktor TC. Storage of spatial information by the maintenance mechanism of LTP. Science. 2006 Aug. 313: 1141-4.
- Properties
  - Alternative Name
    
    z-Pseudosubstrate inhibitory peptide
    
    CAS Number
    
    908012-18-0
    
    Molecular Formula
    
    C90H154N30O17
    
    Molecular Weight
    
    1928.4
    
    Appearance
    
    A solid
    
    Purity
    
    99.00%
    
    Solubility
    
    ≥192.8 mg/mL in DMSO; insoluble in EtOH; ≥2.18 mg/mL in H2O with gentle warming and ultrasonic
    
    Storage
    
    Store at -20°C
    
    * For Research Use Only

ZIP (SCRAMBLED)

Background

We Also Recommend

SAR405

THZ1

WZ3146