Z-Glutamyl(γ-hydroxamate)-glycine

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Background

The standard hydroxamate assay uses Z-Gln-Gly-OH as peptidic glutamine substrate and hydroxylamine as amine donor. In the presence of MTG, hydroxylamine is enzymatically incorporated into the peptide to form Z-Glutamyl(γ-hydroxamate)-glycine. The hydroxamate forms a red colored complex with iron (III) ions quantified at 525 nm.One unit of microbial transglutaminase activity is defined as the amount of enzyme, which causes the formation of 1.0 μmole of hydroxamate per minute at 37ºC (Folk and Cole, 1966).Z018 represents the reaction product to be measured by the chromogenic endpoint assay, allowing the determination of a calibration curve. For each setting, molar attenuation coefficient (ε) needs to be determined individually.We recommend using Z018 to replace G048, which represents the glutamyl(γ-hydroxamate) as a surrogate only.