WizPure™ MMLV RTase (RNase H+)

WizPure™ MMLV RTase (RNase H+)

Catalog Number:
PER1059435WIZ
Mfr. No.:
W2301(-5)
Price:
$200
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      • Overview
        • WizPure™ MMLV RTase (RNase H+) encoded by Moloney Murine Leukemia Virus (MMLV) is an RNA-dependent DNA polymerase that synthesizes the complementary cDNA first strand from a single-stranded RNA template to which a primer has been hybridized.
          This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates. MMLV RTase will also extend primers hybridized to single-stranded DNA. Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase.

          Overview
          • 5' to 3' exonuclease: No
          • 3' to 5' exonuclease: No
          • 3’ – A Overhang: No
          • Fragment Size: Up to 10 kb
          • Free of endo- and exodeoxyribonucleases, phosphatases, and ribonuclease
          • 200 units/μl

          Features
          10,000 U contains:
          1 x 50 μl, MMLV RTase (RNase H+)
          1 x 500 μl, 5X Reaction Buffer

          50,000 U contains:
          1 x 250 μl, MMLV RTase (RNase H+)
          1 x 2,500 μl, 5X Reaction Buffer

          Please contact us at for specific academic pricing.

      • Properties
        • Storage
          Store at -20℃ in a frost-free freezer
          Shipping
          Shipped on ice packs and/or dry ice

          * Research use only

      • Applications
        • Application Description
          Gene synthesis
          First-strand synthesis of cDNA
          RT-PCR
          Random priming reactions
          Library construction
          Probe labeling
          mRNA 5’-end mapping

    Documents

    Protocol

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