WizScript™ III Reverse Transcriptase

WizScript™ Reverse Transcriptase is a genetically modified M-MLV Reverse Transcriptase that exhibits RNA or DNA-dependent DNA polymerase but lacks ribonuclease H activity.
This enzyme can synthesize a complementary DNA strand initiating from a primer using RNA or DNA templates. Removal of the RNase H activity results in an increase of full-length cDNA products. The RNase H activity degrades the RNA part of the cDNA/RNA hybrid following reverse transcription, which may prevent its inhibition during subsequent PCR steps.

Overview
• RNase H negative
• Molecular weight : 71 kDa
• Reaction temperature : 37°C ~ 42°C
• Heat inactivation : 70°C / 10 min.
• Free of endo- and exodeoxyribonucleases, phosphatases, and ribonuclease
• 200 units/μl

Features
10,000 U contains:
1 x 50 μl, WizScript™ III Reverse Transcriptase
1 x 500 μl, 5X Reaction Buffer [250 mM Tris-HCl, 375 mM KCl, 15 mM MgCl2, 50 mM dithiothreitol, pH 8.3 (25°C)]

50,000 U contains:
5 x 50 μl, WizScript™ III Reverse Transcriptase
5 x 500 μl, 5X Reaction Buffer

Please contact us at for specific academic pricing.