Wheat Germ Coated - 96 well Solid plates

Wheat Germ Plates 96-Well Solid Plates: Solid format designed for high-performance assays.

Wheat Germ Plates are designed for their specific affinity to molecules containing N-acetyl-D-glucosamine residues. The surface of these plates enables precise binding of the carbohydrate fractions of glycoproteins, enzymes, and cell membranes, making them ideal for targeted assays. Derived from the common wheat germ (Triticum vulgaris), Wheat Germ Lectin is a hemagglutinin widely used for isolating glyco-conjugates and glycoproteins with specific carbohydrate structures.

Key Applications of Wheat Germ Coated Plates include studying the surfaces of normal and transformed cells, purifying glycoproteins such as membrane-associated glycoproteins, and investigating changes in cell surface structures during development and the cell cycle. These applications make the plates an essential tool for research in cell biology and glycobiology, offering precise and reliable binding capabilities for complex carbohydrate structures.

Wheat Germ Coated Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.

Wheat Germ Coated 96 Well Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.

Surface:
The Wheat Germ Coated Surface provides a highly sensitive and effective platform for specifically binding the carbohydrate fractions of glycoproteins, enzymes, and cell membranes. This coating is especially useful in applications such as studying the surface characteristics of normal and transformed cells, analyzing cell surface changes throughout development and the cell cycle, and purifying glycoproteins, including membrane-associated glycoproteins. The wheat germ lectin binds selectively to N-acetylglucosamine and sialic acid residues, making these plates ideal for research in cell biology, glycobiology, and membrane protein analysis, where precise and targeted binding is essential for accurate results.

Key features of Wheat Germ Plates
· Wheat Germ Coated surface: It enables precise binding of the carbohydrate fractions of glycoproteins, enzymes, and cell membranes and it is suitable for:
· Studies of surfaces of normal and transformed cells
· Glycoprotein purification including membrane glycoproteins
· Studies of cell surface changes during development and the cell cycle
· Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
· Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
· Wells design: flat bottom.
· Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
· Colors: Available in clear, white, and black. Customizable upper rim colors upon request.
Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
· Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
· Alphanumeric Coding: For easy and accurate well identification during experiments.
· Recommended working volume: of 50 to 100 μl
· Packaging: Each coated plate is packed in a single barrier bag with desiccant
· Unit: Contains 5 plates
· Minimum order: 10 plates.
· Ready to use

Key Benefits
· Plates Design:
The mould design provides optical quality, important to reduce the background signal.
The rim protects the external face of the bottom from scratches.
· Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
· Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
· Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
· Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Please contact us at for specific academic pricing.

Background

Wheat Germ Coated Surface offers a powerful and sensitive instrument for binding in specific way the carbohydrate fraction of glycoproteins, enzymes and cell membranes.
Wheat Germ Coated Surface shows specific affinity for molecules containing N-acetyl-D-glucosamine residue and binds in specific way the carbohydrate fraction of glycoproteins, enzymes and cell membranes.

Wheat Germ Lectin, belonging to the lectins family, is a Hemagglutinin obtained from the common wheat germ Triticum Vulgaris. It is well known that lectins have been used extensively for the isolation of glyco-conjugates and glycoproteins with specific carbohydrate structures.

The optical properties of polystyrene remain unchanged, allowing the use of the modified surface as a powerful tool for diagnostic assays.

· Examples of applications for Wheat Germ Coated surface
studies of surfaces of normal and transformed cells
glycoprotein purification including membrane glycoproteins
studies of cell surface changes during development and the cell cycle

· Wheat Germ Coated Surfaces main features
Biomat Wheat Germ Coated products have the following characteristics:

Ready to Use
Manufactured under ISO:9001 guidelines
All lots are tested for uniformity and reproducibility
Certificate of Quality is released for every lot
For Research Use Only
Also, Wheat Germ Coated Products ensures:

Uniformity
The Wheat Germ Coated Products show a CV% less than 5 when a HIgG-HRP is used as detector and TMB as substrate in an ELISA format.

Storage and Stability
The Wheat Germ Coated products, if unopened, are stable at 2 – 8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

· Wheat Germ Coated Microplates Specifications
Coating
Wheat Germ is coated using 200 μl/well.

Binding specificity
Biomat has evaluated the binding specificity of its Wheat Germ Coated 96-Well Plate. Click on the following link to see the results.