Name: Vacuolin-1
Price: 266.00 USD

- Overview
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    Background
    
    Vacuolin-1 is a potent and cell-permeable inhibitor of Ca2+-dependent lysosomal exocytosis [1].
    Exocytic fusion of lysosomes triggered by plasma membrane damage is the major source of the membrane required for resealing. Lysosomal markers appear at the cell surface or are released into the medium on transient elevation of cytosolic Ca2+, including that induced by plasma membrane disruption [1].
    Vacuolin-1 is a cell-permeable blocker of Ca2+-dependent lysosomal exocytosis induced by ionomycin or plasma membrane wounding. In HeLa cells, 5 or 10 μM Vacuolin-1 reduced the release of lysosomal β-hexosaminidase. Vacuolin-1 also blocked the ionomycin-induced, Ca2+-dependent cell surface appearance of the luminal epitope from the lysosomal membrane protein, Lamp-1, a marker for fusion between the limiting membranes of lysosomes and the cell surface. Thus, vacuolin-1 blocks the Ca2+-dependent fusion of lysosomes with the plasma membrane and the release of lysosomal contents. While vacuolin-1 had no effect on the fusion of enlargeosomes with the plasma membrane. Other cell structures and membrane trafficking functions were also unaffected [1].
- Properties
  - Alternative Name
    
    3-iodo-2-[4-(diphenylamino)-6-(4-morpholinyl)-1,3,5-triazin-2-yl]hydrazone, benzaldehyde
    
    CAS Number
    
    351986-85-1
    
    Molecular Formula
    
    C26H24IN7O
    
    Molecular Weight
    
    577.4
    
    Appearance
    
    A crystalline solid
    
    Purity
    
    Purity ≥95.00%
    
    Solubility
    
    ≥7.28 mg/mL in DMSO with ultrasonic; insoluble in EtOH; insoluble in H2O
    
    Storage
    
    Store at -20°C
    
    * For Research Use Only
- Reference
  - 1. Liu B, Cao W, et al. "Lysosomal exocytosis of ATP is coupled to P2Y(2) receptor in marginal cells in the stria vascular in neonatal rats." Cell Calcium. 2018 Sep 21;76:62-71. PMID:30273839