UltraGRO™-Advanced Fibrinogen depleted cell culture supplement - Research grade

UltraGRO™-Advanced cell culture supplement is a fibrinogen depleted, non-xenogeneic (original material), animal serum-free supplement for replacing FBS (fetal bovine serum) to support cell expansion from research use. UltraGRO™-Advanced contains abundant growth factors and cytokines necessary for research or industrial cell growth and proliferation of multiple cell types (e.g. MSCs) in research-scale production.

Instructions for Use:
1. UltraGRO™-Advanced shows optimal growth of MSC at 5% (v/v) in typical cell culture media, i.e. α-MEM, which contains 2mM L-Glutamine as final concentrate.
2. We recommend seeding MSCs at approximately 3x10³~6x10³ per cm².
3. UltraGRO™-Advanced has been fibrinogen-depleted and does not require the addition of heparin in the cell culture media.

Cell Lines:
Bone marrow mesenchymal stem cells, adipose tissue derived mesenchymal stem cells, umbilical cord derived mesenchymal stem cells, other mesenchymal stem cells.

Important Information:
1. Insoluble particles may form in thawed UltraGRO™-Advanced. Published research has shown that particles will not alter the performance of the product.
2. Insoluble particles may form in thawed UltraGRO™-Advanced, it is recommended to centrifuge at 3,400 xg for 3~5 minutes.
3. Filtering the completed medium (e.g. 5%), after UltraGRO™-Advanced is diluted in the basal medium, will not affect UltraGRO™-Advanced supplemented cell culture performance. However, 0.22 μm filtering is NOT recommended for the 100% UltraGRO™-Advanced concentrate, as this may reduce 5% UltraGRO™ -Advanced cell culture performance.
4. Repeated freeze-thaw cycles should be avoided as they will cause an increase in insoluble precipitates and resulting potential decrease in UltraGRO™-Advanced performance.

Please contact us at for specific academic pricing.