UltraGRO™ Cell culture supplement - GMP grade

UltraGRO™ cell culture supplement is a xeno-free, animal serum-free, and heparin-requiring supplement for replacing FBS (fetal bovine serum) to support cell expansion from research and clinical trials to commercial application . UltraGRO™ contains abundant growth factors and cytokines necessary for research or industrial cell growth and proliferation of multiple cell types (e.g. MSCs) in research-scale or industrial-scale production.

Instructions for Use:
1. UltraGRO™ shows optimal growth of MSC at 5% (v/v) in typical cell culture media, i.e. α-MEM, which contains 2mM L-Glutamine as final concentrate.
2. We recommend seeding MSCs at approximately 3x10³~6x10³ per cm².
3. UltraGRO™ requires heparin at a final concentration of 2 IU/mL to be added in the culture media when supplemented with 5% UltraGRO™. Failure to add heparin will result in coagulation during cell culture in typical media.

Cell Lines:
Bone marrow mesenchymal stem cells, adipose tissue derived mesenchymal stem cells, umbilical cord derived mesenchymal stem cells, other mesenchymal stem cells

Important Information:
1. Clotting or insoluble particles may form in thawed UltraGRO™. Published research has shown that particles will not alter the performance of the product.
2. Clotting or insoluble particles may form in thawed UltraGRO™, it is recommended to centrifuge at 3,400 xg for 3~5 minutes or to filter the liquid concentrate with a sterile 40 μm Cell Strainer to remove insoluble particles.
3. Filtering the completed medium (e.g. 5%), after UltraGRO™ is diluted in the basal medium, will not affect UltraGRO™ supplemented cell culture performance. However, 0.22 μm filtering is NOT recommended for the 100% UltraGRO™ concentrate, as this may reduce 5% UltraGRO™ cell culture performance.
4. Repeated freeze-thaw cycles should be avoided as they will cause an increase in insoluble precipitates and resulting potential decrease in UltraGRO™ performance.

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