UltraGRO™-PURE GI Gamma irradiated fibrinogen depleted cell culture - GMP grade

UltraGRO™-PURE GI cell culture supplement is a gamma irradiated viral inactivated, fibrinogen depleted, xeno-free media supplement for replacing FBS (fetal bovine serum) to support cell expansion in research, clinical trials and commercial applications. UltraGRO™-PURE GI contains abundant growth factors and cytokines necessary for cell growth and proliferation of multiple cell types (e.g. MSCs) in research-scale or industrial-scale production.

Instructions for Use:
1. UltraGRO™-PURE GI shows optimal growth of MSC at 5% (v/v) in typical cell culture media, i.e. α-MEM, which contains 2mM L-Glutamine as final concentrate.
2. We recommend seeding MSCs at approximately 3x10³~6x10³ per cm².
3. UltraGRO™-PURE GI has been fibrinogen-depleted and does not require the addition of heparin in the cell culture media.

Cell Lines:
Bone marrow mesenchymal stem cells, adipose tissue derived mesenchymal stem cells, umbilical cord derived mesenchymal stem cells, other mesenchymal stem cells

Important Information:
1. Insoluble particles may form in thawed UltraGRO™-PURE GI. Published research has shown that particles will not alter the performance of the product.
2. Insoluble particles may form in thawed UltraGRO™-PURE GI, it is recommended to centrifuge at 3,400 xg for 3~5 minutes to remove particles prior to use.
3. Filtering the completed medium (e.g. 5%), after UltraGRO™-PURE GI is diluted in the basal medium, will not affect UltraGRO™-PURE GI supplemented cell culture performance. However, 0.22 μm filtering is NOT recommended for the 100% UltraGRO™-PURE GI concentrate, as this may reduce 5% UltraGRO™-PURE GI cell culture performance.
4. Repeated freeze-thaw cycles should be avoided as this will cause an increase in insoluble precipitates and resulting potential decrease in UltraGRO™-PURE GI performance.

Please contact us at for specific academic pricing.