UCF.ME® T4 Polynucleotide Kinase (10 U/μL)

T4 Polynucleotide kinase is a polynucleotide 5'-hydroxy kinase that can catalyze the transfer of the γ-phosphate group of ATP to the 5'-hydroxyl end of the oligonucleotide chain (double-stranded or single-stranded DNA or RNA) and the 3'-monophosphate nucleoside, and the reaction is reversible. T4 Polynucleotide kinase also has 3'-terminal phosphatase activity, hydrolyzing the 3'-phosphate group from the 3'-phosphate end of the oligonucleotide, deoxy 3'-monophosphate nucleoside and deoxy 3'-diphosphate nucleoside. When ADP is present, T4 PNK has 5'-terminal phosphatase activity, catalyzing the exchange of 5'-P-oligo/polynucleotide and ATP terminal 5'-phosphate groups, which is suitable for DNA library construction and end-labeling probe production. Compared with T4 Polynucleotide kinase, UCF.ME® T4 polynucleotide Kinase has lower host residues and is suitable for application scenarios with more stringent requirements on background bacteria, such as pathogenic microorganism detection.

Components: UCF.ME® T4 polynucleotide Kinase (10 U/μL) (1 mL); 10× T4 PNK Buffer (2 × 1 mL)

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