Hieff Canace®II High-Fidelity DNA Polymerase

Hieff Canace®II High-Fidelity DNA Polymerase is a new generation of high-fidelity DNA polymerase. The enzyme is based on Pyrococcus Furiosis DNA Polymerase and is genetically engineered. Its protein structure contains a 5'→3' polymerase domain and a 3'→5' exonuclease domain. This combination greatly improves the heat resistance and fidelity of the enzyme. The heat resistance temperature reaches 98°C, and the fidelity is 52 times that of Taq DNA polymerase and 6 times that of ordinary Pfu DNA polymerase. The enzyme is mainly designed for NGS library amplification, overcoming the GC-bias caused by amplifying templates with different GC contents. At the same time, the amplification efficiency is 1.5 times higher than that of the first generation Canace, and the library amplification yield is greatly increased. This product is equipped with a separate buffer and a special amplification promoter, with extremely high amplification efficiency and wide template adaptability.

Activity definition:
Using activated salmon sperm DNA as template/primer, the activity of 10 nmol of total nucleotides taken up as acid-insoluble matter at 74°C for 30 min is defined as 1 activity unit (U).

Components: Hieff Canace®II High-Fidelity DNA Polymerase (1 U/μL) (100 μL/500 μL); 2×Canace®II PCR buffer (containing Mg2+, dNTPs) (3×1 mL/15×1 mL)

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