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Ubiquitin 7-amido-4-methylcoumarin (Ub-AMC)

Ubiquitin 7-amido-4-methylcoumarin (Ub-AMC)

Catalog Number:
UULP097274UBP
Mfr. No.:
M3011
Price:
$1,425
  • Size:
    5×50µg
    Quantity:
    Add to Cart:
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      • Overview

        • Ubiquitin 7-amido-4-methylcoumarin (Ub-AMC) is a sensitive fluorescence-based substrate of deubiquitinating enzymes. It is useful for monitoring deubiquitinating enzymes’ activity or screening inhibitors of deubiquitinating enzymes. The concentration of Ub-AMC used in deubiquitinating reactions is usually 0.1-1 µM. The released AMC fluorescence can be monitored using a fluorometer or plate reader with excitation and emission wavelengths at 360 nm and 460 nm, respectively.

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      • Properties
        • Protein Name
          Ubiquitin-AMC
          Source
          Synthetic
          Formulation
          20 mM Tris, pH 7.1 at 37 0C, 150 mM NaCl, 2 mM βME, 10% Glycerol
          Storage
          Store at -80°C; avoid multiple freeze-thaw cycles
          Concentration
          See tube label
          Molecular Weight
          9 kDa

          * Research Use Only

    Datasheet

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