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Coomassie-stained SDS-PAGE. Lane 1: Molecular weight markers; Lane 2: Ub-Rhodamine 110

Activity assay of Ub-Rhodamine 110 (100 nM) with varying concentrations UCH-L3 (5 - 313 pM).

Reaction buffer: 20 mM Tris, pH 7.1 at 37°C, 150 mM NaCl, 2 mM DTT. Reactions contained 0.5 μM Ub-Rhodamine 110 alone (control, brown line), or with 10 nM GST-Usp15 (gray line) or with 20 nM GST-Usp15 (yellow line). Rhodamine110 fluorescence was monitored by using a plate reader with excitation/emission filters at 485/20 and 530/30 nm, respectively.

Ub-Rhodamine 110

Ub-Rhodamine 110

Catalog Number:

UULP097769UBP

Mfr. No.:

M3020

Price:

$480

Size:

50 µg

Quantity:

Add to Cart:

- Overview
  - Ub-Rhodamine 110 is a quenched, fluorescent substrate for deubiquitinating enzymes. Cleavage of the amide bond between the C-terminal glycine of ubiquitin and rhodamine110 results in an increase in rhodamine fluorescence at 535 nm (Exc. 485 nm).
    
    Please contact us at for specific academic pricing.
- Properties
  - Source
    
    Synthetic
    
    Formulation
    
    20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
    
    Storage
    
    Powder at −20°C; solution at −80°C. Avoid multiple freeze/thaw cycles.
    
    Concentration
    
    N/A
    
    Molecular Weight
    
    8936 Da by MS
    
    * Research Use Only

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