TransStart® FastPfu Fly DNA Polymerase

TransStart® FastPfu Fly DNA Polymerase

Catalog Number:
PER1559231TRA
Mfr. No.:
AP231-21/22/23
Price:
$222
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      • Overview
        • TransStart® FastPfu Fly DNA Polymerase is a hot start, ultra high-fidelity DNA Polymerase used for fast PCR. Compared with TransStart® FastPfu DNA Polymerase, TransStart® FastPfu Fly DNA Polymerase has higher extension rate (≤ 5 kb fragments can achieve 12 kb/min extreme amplification, > 5 kb fragments can achieve 6 kb/min high-speed amplification), featuring higher amplification efficiency, higher yield, higher fidelity and higher sensitivity. 2×TransStart® FastPfu Fly Reaction Mix already contains dNTPs. When DNA is amplified, just add template, primer, water and TransStart® FastPfu Fly DNA Polymerase. The Reaction can be carried out with the concentration of Reaction Mix being 1×. PCR products are blunt end and can be cloned directly into pEASY®-Blunt series of vectors.
          • Offers 10⁸-fold fidelity as compared to EasyTaq® DNA Polymerase.
          • PCR products are blunt end and can be cloned directly into pEASY®-Blunt series of vectors.
          • Amplification of genomic DNA fragment up to 15 kb.
          • Amplification of plasmid DNA fragment up to 20 kb.

          Features
          • Hot start, high specificity.
          • High amplification efficiency.
          • Fast and ultra high-fidelity.
          • High sensitivity.
          • High yield.

          Please contact us at for specific academic pricing.

      • Properties
        • Categories
          DNA Polymerase
          Other Properties
          Components:
          • TransStart FastPfu Fly DNA Polymerase
          • 2x TransStart FastPfu Fly Reaction Mix
          • 50 mM MgSO4
          • 6xDNA Loading Buffer
          • PCR Stimulant
          Storage
          at -18°C or below for two years
          Shipping
          Dry Ice (-70°C)

          * For Research Use Only.

      • Applications
        • Application Description
          • Complex templates, GC/AT-rich templates.
          • Ultra high-fidelity and fast PCR, blunt end cloning, site-directed mutagenesis.
          • Long fragment amplification.
      • Reference
        • 1 Zhang A, Shan T, Sun Y, et al. Directed evolution rice genes with randomly multiplexed sgRNAs assembly of base editors[J]. Plant Biotechnology Journal, 2023. (IF 13.80)
          2 Xu Y, Zhu T F. Mirror-image T7 transcription of chirally inverted ribosomal and functional RNAs[J]. Science, 2022. (IF 63.714)
          3 Wang D, Yan F, Wu P, et al. Global profiling of regulatory elements in the histone benzoylation pathway[J]. Nature Communications, 2022. (IF 14.919)
          4 Niu L, Shen W, Shi Z, et al. Three-dimensional folding dynamics of the Xenopus tropicalis genome[J]. Nature Genetics, 2021. (IF 38.33)
          5 Jin S, Fei H, Zhu Z, et al. Rationally designed APOBEC3B cytosine base editors with improved specificity[J]. Molecular cell, 2020. (IF 15.58)
          6 Chen J, Ou Y, Yang Y, et al. KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing[J]. Nature, 2018. (IF 40.13)

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