Name: TOOS
Price: 299.00 USD

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    New Trinder's Reagents
    
    CAS Number
    
    82692-93-1 (anhydrous)
    
    Molecular Formula
    
    C12H18NNaO4S, 2H2O
    
    Molecular Weight
    
    331.36
    
    Appearance
    
    White or slightly blue powder
    
    Purity
    
    ≥98.0% (Absorbance)
    
    Storage
    
    ambient temperature, protect from moisture and light
    
    Shipping
    
    ambient temperature
    
    * For research use only
- Applications
  - Application Description
    
    Preparation of Assay Solution - TOOS
    1. Dissolve 20 mg TOOS with 10 ml PBS to prepare 6.6 mM TOOS solution.
    2. Dissolve 14 mg 4-aminoantipyrin (AA) with 10 ml PBS to prepare 6.6 mM 4-AA solution.
    3. Prepare 2 U/ml horseradish peroxidase solution with PBS.
    4. Mix the same volume of each solution together to prepare assay solution. Store the assay solution at 4°C with protection from light.
    Assay Protocol
    1. Prepare sample solutions for the enzymatic oxidation reaction. The pH range of the buffer solution should be from 5.5-9.5.
    2. Prepare standard solutions containing known amounts of substrate using the same buffer.
    3. Add the appropriate units of oxidase to the sample solution, followed by an addition of the same volume of the assay solution.
    4. Incubate the mixture at room temperature or at 37°C for 30 min to 1 hour.
    5. Measure the O.D. at 555 nm.
    6. Prepare a standard curve, and determine the substrate concentration in the sample solution.
- Reference
  - 1) K. Tamaoku, et al., New water-soluble Hydrogen Donors for the Enzymatic Photometric Determination of Hydrogen Peroxide. II. N-Ethyl-N-(2-hydroxy-3-sulfopropyl)aniline derivatives. Chem Pharm Bull. 1982;30:2492-2497.
    
    2) K. Tamaoku, et al., New water-soluble Hydrogen Donors for the Enzymatic Spectrophotometric Determination of Hydrogen Peroxide. Anal Chim Acta. 1982;136:121-127.
    
    3) B. C. Madsen, et al., Flow Injection and Photometric Determination of Hydrogen Peroxide in Rainwater with N-Ethyl-N-(sulfopropyl)aniline sodium salt. Anal Chem. 1984;56:2849-2850.

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