Name: SAT-3
Price: 461.00 USD

- Overview
  - SAT-3 is a stable, highly water-soluble o-Tolidine analog. It is easily oxidized by peroxidase and hydrogen peroxide into a green dye at pH 4-6. While TMBZ requires an organic solvent or detergent to solubilize, SAT-3 can be solubilized with just buffer solution. The SAT-3 solution can be stored at room temperature with protection from light. The maximum wavelength of the dye is 675 nm. Using the Ames test, Nno mutagenicity of SAT-3 was detected. using the Ames test.
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Oxidative Chromogenic Dyes
    
    Molecular Formula
    
    C20H26N2Na2O8S2• 4H2O
    
    Molecular Weight
    
    604.62
    
    Appearance
    
    White or pale-brown powder
    
    Purity
    
    ≥95.0% (HPLC)
    
    Storage
    
    0-5°C, protect from light
    
    Shipping
    
    ambient temperature
    
    * For research use only
- Applications
  - Application Description
    
    1. Mix 1 ml of 100 mM SAT-3 solution and 1 ml of 30 mM hydrogen peroxide solution with 8 ml of 50 mM citrate buffer (pH 4) to prepare an assay solution.
    2. Add 100-200 μl of the assay solution to each well of a 96-well plate and incubate at 37°C for 5-30 minutes.
    3. Read the O.D. at 670 nm or add 50 μl of 4 M sulfuric acid to each well and read the O.D. at 490 nm instead.
- Reference
  - M. Mizoguchi, et al., Sensitive Chromogenic Substrate for Detecting Peroxidase Activity. Anal Commun. 1998;35:179.

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