THP-1-cas9 Cell (Human Monocytic Leukemia)

Species: human
Cell Morphology: Suspension cells
Complete Culture Medium Components: 1640 + 10% FBS + 0.05 mM2ME
Mycoplasma testing: Negative
Antibiotic Concentration: puromycin, 0.5ug/ml
Cultivation Environment: 37°C, 5% CO₂
Passage Ratio: 1/2 to 1/4 of the generations
Frequency of Reproduction: 2-3 times/week
Gene Expression: Cas9 protein, spCas9, CRISPR-Cas9 protein

Product Advantages:
• Efficiency and Convenience: A "ready-to-use" platform; simply import gRNA, operation is simple, editing efficiency is high, saving time and costs.
• High-Throughput Screening: An ideal foundational platform for genome-wide CRISPR screening, used for systematic discovery of functional genes.
• Consistency and Reproducibility: Consistent cell population genetic background, stable Cas9 expression, more reliable experimental results, and more rigorous control settings.
• Functional Scalability: Cell lines expressing dCas9, high-fidelity Cas9, and other variants can be constructed to achieve multiple functions beyond gene knockout (regulation, imaging, etc.).

Please contact us at for specific academic pricing.

Background

THP-1 cells phagocytose milk droplets and activated erythrocytes, but lack surface and cytoplasmic immunoglobulins. THP-1 cells can be induced to differentiate into monocytes using phorbol and TPA.