T7 RNA Polymerase, GMP Grade (50U/μl)

- Overview
  - As a biological macromolecule, mRNA can be synthesized on a large scale by in vitro transcription (IVT). T7 promoter is one of the most efficient promoters at present. Therefore, T7 RNA polymerase can be used for in vitro transcription to obtain more synthetic products. T7 RNA polymerase is a T7 promoter-specific, DNA-dependent, 5'→3' RNA polymerase from T7 bacteriophage. Using double stranded DNA as the template, it transcribes RNA complementary to the single stranded DNA located at the downstream of T7 promoter. T7 RNA polymerase has been commonly used for in vitro mRNA synthesis.
    The polymerase is GMP Grade produced in E. coli. Our manufacturing processes are strictly controlled to ensure the end products free from host protein or nucleic acid contaminations and other impurities following the Pharmaceutical Manufacturing Guidelines. We guarantee the manufacturing and quality control comply with GMP regulation for tracking each and every step of the manufacturing process, including raw material sourcing.
    This product has completed the DMF record of FDA and passed the HALAL certification.
    
    Please contact us at for specific academic pricing.
    
    More Details
    
    FDA DMF Filed Enzymes
- Properties
  - Storage
    
    At -20±5 °C.
    
    Expression system
    
    E. coli
    
    Endotoxin
    
    ＜5EU/ml
    
    Activity
    
    49KU/ml-51KU/ml (Activity Definition: At 37 °C, pH 8.0, within 1 hour, the amount of enzyme required to incorporate 1 nmol tritium (3H)) labeled GMP into acid-insoluble material is defined as one unit of enzyme activity.)
    
    * For Research or Manufacturing Use Only, Not for Use in Diagnostic or Therapeutic Procedures.
- Reference
  - Development of an mRNA vaccine against a panel of heterologous H1N1 seasonal influenza viruses using a consensus hemagglutinin sequence
    The development and characterization of a stable Coxsackievirus A16 infectious clone with Nanoluc reporter gene
    An mRNA-based rabies vaccine induces strong protective immune responses in mice and dogs

Datasheet

T7 RNA Polymerase, GMP Grade (50U/μl)

T7 RNA Polymerase, GMP Grade (50U/μl)

More Details

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