T7 High Yield RNA Synthesis Kit for Co-transcription (low dsRNA, with LZCap AG)

The kit utilizes T7 RNA Polymerase (Low dsRNA) and high-affinity LZcap AG Cap1 analog to produce mRNAs with significantly reduced double-stranded RNA (dsRNA) content and enhanced expression. Single-stranded RNAs are synthesized using linear double-stranded DNA containing a T7 promoter sequence as the template. Modified nucleotides, such as biotin or dye-labeled nucleotides, can be incorporated during transcription to generate labeled RNA. The kit supports the synthesis of both long and short transcripts, yielding 100–200 μg of RNA from 1 μg of DNA template. Substituting UTP with N1-Me-Pseudo UTP reduces RNA immunogenicity. The resulting RNA is suitable for downstream applications, including RNA structure and function studies, RNase protection, probe hybridization, RNAi, microinjection, and in vitro translation.

Features
• Complete Kit Components: Includes all essential reagents for one-step co-transcriptional capping, such as NTPs, T7 Enzyme Mix, cap analog, DNase I, and LiCl.
• Simplified Workflow: Utilizes a co-transcriptional capping process, minimizing the number of components introduced and streamlining the overall procedure for ease of use.
• High Yield: Generates 150–200 µg of Cap 1 mRNA from just 1 µg of DNA template.
• Reduced Immunogenicity: Incorporates modified N1-Me-pUTP and a low dsRNA T7 RNA polymerase to minimize unintended innate immune responses.
• High Capping Efficiency: Achieves capping efficiency exceeding 99%.

Please contact us at for specific academic pricing.