T-VDA GPCR

Catalog Number:
TVDA089649VEN
Mfr. No.:
T-VDAGPCR
Price:
  • Size:
    12 species, 2D HPLC, 1mg protein/fraction
    Quantity:
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      • Overview
        • Although not typically expected as a pathway for venoms, GPCR modulation has been discovered in several snake venoms including muscarinic acetylcholine receptor blockers. Snake venoms are a rich source of GPCR tools such as the three-finger toxin motif that is particularly effective at binding GPCRs. These targeted arrays contain pure venom fractions from 12, 24, 48 or 96 species optimised for identification of novel tools. Each array contains characterised venoms active in GPCR pathways from the literature to act as positive controls. The control venoms for T-VDA GPCR include Crotalus atrox (western diamondback rattlesnake) where the bradykinin B2 receptor antagonist has been discovered [1]; Dendroaspis angusticeps (eastern green mamba) where several novel muscarinic receptor antagonists have been discovered [2]; and Naja kaouthia (monocled cobra) venom which contains a large abundance of three-finger proteins including antagonising nicotinic and muscarinic nicotine receptors [3]. Other venom fractions making up the library have been specially selected by our drug discovery scientists to maximise novel hit potential.
          • Venoms are supplied lyophilised in Echo qualified acoustic source plates (Labcyte Inc) and are useable on any SBS footprint liquid handling device or by hand.
          • 384-well format has 200ng venom fraction per well, suggested dilution 20µl as hit fractions are typically active at 5µg/ml and below.
          • 1536-well format also available.

          Please contact us at  for specific academic pricing.

          Background

          1. Calvete J.J., Fasoli E., Sanz L., Boschetti E., Righetti P.G. (2009). Exploring the venom proteome of the western diamondback rattlesnake, Crotalus atrox, via snake venomics and combinatorial peptide ligand library approaches. J. Proteome Res. 8:3055-3067
          2. Max S.I., Liang J.-S., Potter L.T. (1993). Purification and properties of m1-toxin, a specific antagonist of m1 muscarinic receptors. J. Neurosci. 13:4293-4300
          3. Utkin Y.N., Kukhtina V.V., Kryukova E.V., Chiodini F., Bertrand D., Methfessel C., Tsetlin V.I. (2001). 'Weak toxin' from Naja kaouthia is a nontoxic antagonist of alpha 7 and muscle-type nicotinic acetylcholine receptors. J. Biol. Chem. 276:15810-15815

          Target Information

          Target Name
          G-protein coupled receptors
      • Properties
        • Storage
          Recommend storage at -20°C upon receipt. Lyophilised T-VDAs™ are shipped at ambient temperature, as they are stable for several weeks.
      • Applications
        • Application Description
           T-VDAs™ can be used in any biochemical or cell based assay like any other compound array. The fractionated T-VDAs perform well in Phenotypic assays.
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