T-VDA ASIC

Catalog Number:
TVDA089636VEN
Mfr. No.:
T-VDAASIC
Price:
  • Size:
    12 species, 2D HPLC, 1mg protein/fraction
    Quantity:
    Add to Cart:
      • Overview
        • The ASIC (Acid Sensing Ion Channel) Targeted Venom Discovery Array™ (T-VDAASIC) is specifically designed to maximise discovery of new tools. ASIC channels are important drug targets for neurological disorders, specifically pain. ASIC channel tools from theraphosids (tarantulas) and snakes are the most potent and selective agents currently known. These targeted arrays contain pure venom fractions from 12, 24, 48 or 96 species optimised for identification of novel tools. Each array contains characterised venoms active at ASIC channels from the literature to act as positive controls. The control venoms for T-VDAASIC include Psalmopoeus cambridgei (Trinidad chevron tarantula) which contains Psalmotoxin, a selective blocker of ASIC1a channels [1]; Dendroaspis polylepis (Black mamba) venom which contains Mambalgins that block ASIC1a/2a heteromers [2]; and Dendroaspis angusticeps (Eastern green mamba) venom which contains mambalgin-3 that blocks ASIC1a and ASIC1b channels as well as 1a/2b heteromers [3]. Other venom fractions making up the library have been specially selected by our drug discovery scientists to maximise novel hit potential.
          • Venoms are supplied lyophilised in Echo qualified acoustic source plates (Labcyte Inc) and are useable on any SBS footprint liquid handling device or by hand.
          • 384-well format has 200ng venom fraction per well, suggested dilution 20µl as hit fractions are typically active at 5µg/ml and below.
          • 1536-well format also available.

          Please contact us at  for specific academic pricing.

          Background

          1. Escoubas P., de Weille J.R., Lecoq A., Diochot S., Waldmann R., Champigny G., Moinier D., Menez A., Lazdunski M. (2000). Isolation of a tarantula toxin specific for a class of proton-gated Na+ channels. J. Biol. Chem. 275:25116-25121
          2. Diochot S., Baron A., Salinas M., Douguet D., Scarzello S., Dabert-Gay A.-S., Debayle D., Friend V., Alloui A., Lazdunski M., Lingueglia E. (2012). Black mamba venom peptides target acid-sensing ion channels to abolish pain. Nature 490:552-555
          3. Schweitz H., Diochot S., Baron A., Salinas M., Lingueglia E. (2013). Venom toxins in the exploration of molecular, physiological and pathophysiological functions of acid-sensing ion channels. Submitted (FEB-2013) to UniProtKB C0HJB0

          Target Information

          Target Name
          Acid Sensing Ion Channels
      • Properties
        • Storage
          Recommend storage at -20°C upon receipt. Lyophilised T-VDAs™ are shipped at ambient temperature, as they are stable for several weeks.
      • Applications
        • Application Description
           T-VDAs™ can be used in any biochemical or cell based assay like any other compound array. The fractionated T-VDAs perform well in Phenotypic assays.
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