T-VDA Enz

Enzymes are incredibly useful tools in a wide range of disciplines and industrial processes. Snake venoms are a rich source of enzymes such as phospholipases (PLA2), snake venom metalloproteinase (SVMP), phosphodiesterases, L-amino-acid oxidase (LAO) and many more. Moreover, invertebrate venoms also often contain enzymes such as phospholipase. Along with useful enzymes, venoms contain many enzyme inhibitors of pharmaceutical utility. These targeted arrays contain pure venom fractions from 12, 24, 48 or 96 species optimised for identification of novel tools. Each array contains characterised venoms active in analgesic pathways from the literature to act as positive controls. The control venoms for T-VDA Enz include phospholipase A2-containing Naja nigricollis venom (black-necked spitting cobra) [1]; Deinagkistrodon acutus (hundred pace pit viper) which contains an L-amino-acid oxidase enzyme that induces apoptosis in HeLa cancer cells [2]; and Crotalus adamanteus (Eastern diamondback rattlesnake) venom which contains snake venom metalloproteinases such as Adamalysin [3]. Other venom fractions making up the library have been specially selected by our drug discovery scientists to maximise novel hit potential.
• Venoms are supplied lyophilised in Echo qualified acoustic source plates (Labcyte Inc) and are useable on any SBS footprint liquid handling device or by hand.
• 384-well format has 200ng venom fraction per well, suggested dilution 20µl as hit fractions are typically active at 5µg/ml and below.
• 1536-well format also available.

Please contact us at for specific academic pricing.

Background

1. Stefansson S., Kini R.M., Evans H.J. (1990). The basic phospholipase A2 from Naja nigricollis venom inhibits the prothrombinase complex by a novel nonenzymatic mechanism. Biochemistry 29:7742-7746
2. Zhang L., Wei L. (2007). ACTX-8, a cytotoxic L-amino acid oxidase isolated from Deinagkistrodon acutus snake venom, induces apoptosis in Hela cervical cancer cells. J. Life Sci. 80:1189-1197
3. Gomis-Rueth F.-X., Meyer E.F., Kress L.F., Politi V. (1998). Structures of adamalysin II with peptidic inhibitors. Implications for the design of tumor necrosis factor alpha convertase inhibitors. Protein Sci. 7:283-292