SuperSilencing shRNA Expression Vector Set

When a validated siRNA sequence (for example, obtained by a large-scale siRNA validation screening) needs to maintain gene silencing for a longer period of time, we recommend the use of a short-hairpin RNA (shRNA) vector system. A shRNA expression vector with an incorporated antibiotic resistance gene can: 1) continuously produce shRNAs inside the cell, and 2) allow for selection of transfected cells. Our company is committed to providing the most advanced and convenient shRNA related tools. We recently introduced a new generation highly efficient, ready to use shRNA expression plasmids: our SuperSilencing shRNA Expression Vector System achieves excellent suppression of the target gene, lasting for several weeks or longer.

Our SuperSilencing shRNA Expression Vector characteristics:
- Stable shRNA expression to achieve continuous RNAi.
- Screening and selectable markers help to establish a stably transfected cell line.
- Two cloning restriction sites: BamH I and BbsI. BbsI is a special restriction endonuclease that produces asymmetrical complementary sticky ends to ensure correct insertion of the target DNA oligo, and prevents self-circularization of the plasmid vector.
- Convenient and easy to operate.
- Economical: one only needs to synthesize DNA oligos against the target gene.

Our company can construct ten kinds of shRNA expression vectors, with or without selectable markers, based on two different plasmids:
pGPU6, pGPH1, pGPU6/Neo, pGPH1/Neo, pGPU6/Hygro, pGPH1/Hygro, pGPU6/GFP/Neo, pGPH1/GFP/Neo, pGPU6/RFP/Neo, pGPH1/RFP/Neo;
- pGPU6, pGPH1: shRNA expression plasmids; Selectable and screening markers:
- Neo: Neomycin (Kan/G418) resistance gene
- Hygro: Hygromycin B resistance gene
- GFP: GFP reporter gene
- RFP: RFP reporter gene

Our company's SuperSilencing shRNA Expression Vector Set:
Each set contains 4 linear or cyclic shRNA expression vectors designed for stable RNAi knockdown of your target gene: we guarantee that at least one of them causes at least 70% inhibition efficiency at the mRNA level. Each set also includes the corresponding negative control vector and a GFP positive control vector.

Please contact us at for specific academic pricing.