Sulfo-NHS-Biotin

- Overview
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    Background
    
    Sulfo-NHS-Biotin (N-Hydroxysulfosuccinimidobiotin) is an amine-reactive biotinylation reagent. The most common amine-reactive biotinylation reagents can be divied into two basic types, N-hydroxysuccinimide (NHS) esters and carboxylates. Generally, NHS esters biotinylation reagent contains a reactive NHS ring structure that reacts with an amine on the carbonyl group of a protein or other molecules through nucleophilic attack subsequently forming a stable amide linkage and releasing the NHS group. Due to the insolubility of NHS-biotin in aqueous environments, sulfo-NHS-biotin, a water-soluble analog of NHS-biotin, has been developed by attaching a negatively charged sulfonate group on the NHS ring structure, which can be added directly to aqueous reactions without the need for organic solvent dissolution.
    Reference
    Bioconjugate Techniques , 2nd ed. By Greg T.Hermanson (Pierce Biotechnology, Thermo Fisher Scientific, Rockford, IL). Academic Press (an imprint of Elsevier): London, Amsterdam, Burlington, San Diego . 2008. ISBN 978-0-12-370501-3.
- Properties
  - Categories
    
    Amine-reactive biotinyltation reagent, water soluble
    
    Alternative Name
    
    Sulfo-NHS Biotin
    
    CAS Number
    
    119616-38-5
    
    Molecular Formula
    
    C14H18N3NaO8S2
    
    Molecular Weight
    
    443.4
    
    Appearance
    
    A solid
    
    Purity
    
    98.00%
    
    Solubility
    
    ≥22.17 mg/mL in DMSO; insoluble in EtOH; ≥59.9 mg/mL in H2O with ultrasonic
    
    Storage
    
    Desiccate at -20°CThe product is not stable in solution, please dissolve it immediately before use.
    
    SMILES
    
    C1C(C(=O)N(C1=O)OC(=O)CCCCC2C3C(CS2)NC(=O)N3)S(=O)(=O)[O-].[Na+]
    
    * For Research Use Only
- Reference
  - 1. Nathan J. Dwarshuis, Hannah W. Song, et al. "Functionalized microcarriers improve T cell manufacturing by facilitating migratory memory T cell production and increasing CD4/CD8 ratio." bioRxiv. 2019 May 23.
    2. Lambert Yue, Christine Sam, et al. "Antibody microarray and immunoblotting analyses of the EGF signaling phosphorylation network in human A431 epidermoid carcinoma cells." Clinical Proteomics & Bioinformatics.2017.