Streptavidin High Binding Coated - 96 well plates - 8 Well Breakable Strips on single well holding frame

Streptavidin High Binding Plates 96-Well Breakable Strip Plates: Composed of 12 breakable strips of 8 wells, these plates allow easy customization by breaking and repositioning strips or single wells as required.

Streptavidin High Binding Coated Plates are ideal for capturing low molecular weight proteins or probes in ELISA assays. The high-binding surface provides a robust platform for immobilizing a wide range of biotinylated molecules, including antibodies, antigens, proteins, peptides, polysaccharides, oligonucleotides, and DNA fragments. This surface is particularly advantageous for molecules that do not adhere well through passive adsorption or that require a specific orientation for optimal binding, ensuring enhanced assay performance. Compared to standard Streptavidin Coated Plates it offers superior sensitivity and accuracy when measuring biotinylated low molecular weight molecules. This makes it a valuable tool for applications demanding high precision and reliable detection.

Streptavidin High Binding Coated Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.

Streptavidin High Binding Coated 96 Well Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.

Surface:
The Streptavidin High Binding Coated Surface provides an exceptionally effective and versatile platform for capturing a wide range of biotinylated molecules, including proteins, peptides, polysaccharides, oligonucleotides, and DNA fragments. This surface is especially valuable for molecules that do not adhere well through passive adsorption or that require precise orientation for optimal binding. Compared to standard streptavidin-coated surfaces, the high-binding variant is particularly suited for indirect assays, enabling the accurate measurement of biotinylated low molecular weight molecules. This increased binding capacity ensures greater sensitivity and stability, making it ideal for assays where high performance and precision are critical.

Key features of Streptavidin High Binding Plates
· High Binding Streptavidin vs. Streptavidin coated surface: particularly suited for measurement of biotinylated low molecular weight molecules.
· Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
· Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
· Wells design: flat bottom.
· Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
· Colors: Available in clear, white, and black. Customizable upper rim colors upon request.
Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
· Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
· Alphanumeric Coding: For easy and accurate well identification during experiments.
· Recommended working volume: of 50 to 100 μl
· Packaging: Each coated plate is packed in a single barrier bag with desiccant
· Unit: Contains 5 plates
· Minimum order: 10 plates.
· Ready to use

Key Benefits
· Plates Design:
The mould design provides optical quality, important to reduce the background signal.
The rim protects the external face of the bottom from scratches.
· Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
· Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
· Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
· Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Please contact us at for specific academic pricing.

Background

Streptavidin High Binding Coated Surface offers a powerful and universal instrument for binding any biotinylated molecule, mostly for measuring biotinylated low molecular weight molecules.
Streptavidin High Binding Coated Surface is suitable for binding antibodies or probes for ELISA assays and other target-specific assays. This surface is a powerful and universal instrument for binding any biotinylated molecule (Proteins-Peptides-Polysaccharides-Oligonucleotides-DNA fragments-etc.).

Biotin is a small molecule which can be conjugated to many proteins without loosing or altering their activity, each protein can bind many biotin molecules.

Since each subunit of streptavidin binds one molecule of biotin, the resulting effect is a great increase of the sensitivity of the assay.

Unlike the normal Streptavidin coated surface, these surfaces are particularly useful to measure biotinylated low molecular weight molecules.

· Streptavidin High Binding Coated Surfaces main features
Biomat Streptavidin High Binding Coated products have the following characteristics:
Ready to Use
Manufactured under ISO:9001 guidelines
All lots are tested for uniformity and reproducibility
Certificate of Quality is released for every lot
For Research Use Only

Also, Streptavidin High Binding Coated Products ensures:

Uniformity
The Streptavidin High Binding Coated Products show a CV% less than 5 using biotin-HRP as detector and TMB as substrate.

Storage and Stability
The Streptavidin High Binding Coated products, if unopened, are stable at 2 – 8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

· Streptavidin High Binding Coated Microplates Specifications
Coating
Streptavidin High Binding is coated using 100 µl/well.

Binding capacity for small molecules (e.g. d-biotin)
The Streptavidin High Binding coated wells were incubated with biotin solutions, in an ELISA format, using biotinylated peroxidase as detector and TMB as substrate.

Binding capacity for big molecules (e.g. HIgG)
The Streptavidin High Binding coated wells were incubated with biotinylated HIgG in an ELISA format using HIgG-HRP as a detector and TMB as substrate.

· Streptavidin High Binding PCR 8 Strip Tubes and PCR Plates Specifications
Coating
Streptavidin High Binding is coated using 100 µl/tube.

Binding capacity for small molecules (e.g. d-biotin)
Streptavidin High Binding coated PCR tubes strips were incubated with biotin solutions containing biotinylated peroxidase for 30' R.T. After the washing step, the PCR tubes strips were incubated with TMB and blocked with sulphuric acid 1N. The O.D. values were read at 450 nm