Neutravidin Coated - 96 well plates - 8 Well Breakable Strips on single well holding frame

Neutravidin Plates 96-Well Breakable Strip Plates: Composed of 12 breakable strips of 8 wells, these plates allow easy customization by breaking and repositioning strips or single wells as required.

Neutravidin Plates are immunoassay plates coated with Neutravidin, specifically designed to bind biotinylated molecules with minimal non-specific binding. These plates are suitable for various applications, including binding biotinylated antibodies, antigens, proteins, peptides, polysaccharides, oligonucleotides, and DNA fragments. Unlike Streptavidin, Neutravidin lacks the RYD sequence (Arg-Tyr-Asp), which eliminates interaction with the RGD sequence (Arg-Gly-Asp) found in cell membrane receptors, making it ideal for assays where cell receptor interference must be avoided. Neutravidin Coated 96-Well Plates are especially useful for molecules that do not reliably bind through passive adsorption or may adsorb in unfavorable orientations, offering a powerful and versatile solution for target-specific assays.

Neutravidin Coated Plates are pre-blocked in order to minimize any non-specific binding and to ensure long-term stability.

Neutravidin Coated Plates are available in clear, white, and black polystyrene in Breakable Strip, Strip and Solid format. They are suitable for ELISA, Luminescence, Fluorescence, and Chemiluminescent assays.

Surface:
The Neutravidin® Coated Surface is designed for the specific binding of biotinylated molecules, such as biotin-tagged antibodies, while minimizing non-specific interactions. This surface provides a highly reliable platform for assays requiring precise biotin-avidin interactions, ensuring accurate and consistent results.

Key features of Neutravidin Plates
· Neutravidin surface: It binds biotinylated molecules, such as biotin-tagged antibodies.
· Blocking: Plates are pre blocked with standard proteic blocking (ELISA Blocking) or with non proteic blocking (BlockerWell)
· Low Fluorescence Polystyrene: Manufactured with pure high-quality polystyrene, minimizing background noise and improving optical clarity.
· Wells design: flat bottom.
· Multiple Formats: Available in breakable strip plates, non-breakable strip plates, and solid plates for flexibility in applications.
· Colors: Available in clear, white, and black. Customizable upper rim colors upon request.
Reduced Crosstalk: White and black plates minimize well-to-well light scatter, improving signal accuracy in luminescence and fluorescence assays.
Uniform Optical Properties: Clear plates are ideal for spectrophotometric readings and imaging, providing consistent and accurate light transmission.
· Automation-Compatible: Easily integrated with high-throughput automated systems for liquid handling, washing, and analysis.
· Alphanumeric Coding: For easy and accurate well identification during experiments.
· Recommended working volume: of 50 to 100 μl
· Packaging: Each coated plate is packed in a single barrier bag with desiccant
· Unit: Contains 5 plates
· Minimum order: 10 plates.
· Ready to use

Key Benefits
· Plates Design:
The mould design provides optical quality, important to reduce the background signal.
The rim protects the external face of the bottom from scratches.
· Improved Washing Efficiency: Radius-edged well bottoms enhance washing, reducing residual materials and improving assay consistency.
· Reduced Cross-Contamination: Designed to minimize the risk of cross-contamination between wells, ensuring precise sample isolation.
· Enhanced Imaging: The flat surface provides a clear focal plane, essential for high-quality imaging in microscopy, especially for fluorescence and phase-contrast analysis.
· Improved Mixing: The flat-bottom design facilitates better sample mixing, ensuring consistent results across assays.

Please contact us at for specific academic pricing.

Background

Neutravidin Coated Surface is ideal to bind any biotinylated molecules at wells of Neutravidin Coated Plates, with a minimal non-specific binding.
Neutravidin is a deglycosylated avidin (M.W. 60 kDa) that contains four identical subunits biotin-binding with very high affinity for biotin (Ka= 10-15 M).

It has an isoelectric point near-neutral (pI=6.3) and the lowest non specific binding properties among the known biotin binding proteins.

Compared to the streptavidin, neutravidin in its primary structure does not contain the RYD sequence (Arg-Tyr-Asp): the lack of such a sequence totally eliminates the possibility to interact with the RGD sequence (Arg-Gly-Asp) present in the membrane receptors of a large variety of cells. This makes it very useful in applications where it is important to avoid the non-specific interaction with the cell surfaces.

Neutravidin Coated Surface has similar binding capacity of Streptavidin Coated Surface for biotin and biotinylated molecules and offers a powerful and universal instrument for binding these molecules minimizing non-specific interactions. For example, Neutravidin Coated Plates are suitable with ELISA assays protocol.

Biomat Neutravidin Coated products
These are the available configurations of Neutravidin Coated products by Biomat.

· Neutravidin Coated Surfaces main features
Biomat's Neutravidin Coated plates have the following characteristics:

Ready to use
Manufactured under ISO:9001 guidelines
Post-coated (blocked) for low non specific binding and long term stability
All lots are tested for uniformity and reproducibility
Certificate of Quality is released for every lot
For Research Use Only
Also, Neutravidin Coated Products ensures:

Uniformity
The Neutravidin Coated plates show a CV% less than 5 using biotin-HRP as detector and TMB as substrate.

Storage and Stability
The Neutravidin Coated products, if unopened, are stable at 2-8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

· Neutravidin Coated Plates product specification
Coating
Neutravidin is coated using 100 µl/well.

Binding capacity for small molecules (e.g. d-biotin)
The Neutravidin coated wells were incubated with biotin solutions, in an ELISA format, using biotinylated peroxidase as detector and TMB as substrate.

Binding capacity for big molecules (e.g. HIgG)
The Neutravidin coated wells were incubated with biotinylated HIgG in an ELISA format using HIgG-HRP as a detector and TMB as substrate.