Stearyl Phosphoramidite

- Overview
  - Synthetic oligonucleotides often require lipophilic and non-toxic modification to carry them into desired cellular targets. The straightforward way to achieve such a modification is the automatic 5'-coupling with an appropriate lipid phosphoramidite. Several publications have shown that this approach is useful for the silencing of gene expression with siRNAs in vivo (Nat. Biotech. 2007, 25, 1149–1157, doi: 10.1038/nbt1339).
    
    We now offer an affordable C18 lipid modifier which is easy to use under the standard synthesis conditions. The obtained 5'-stearyl oligonucleotides can be purified using RP cartridges or standard chromatographic techniques.
    
    Please contact us at for specific academic pricing.
- Properties
  - Alternative Name
    
    Octadecyl-1-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite
    
    Molecular Formula
    
    C27H55N2O2P
    
    Molecular Weight
    
    470.71 g/mol
    
    Appearance
    
    Colorless oil
    
    Other Properties
    
    1H NMR (CD3CN): Conforms to the internal standard
    31P NMR (CD3CN): Conforms to the internal standard
    Assay: 93 % (NMR 31P)
    
    Storage
    
    Keep in a dry place at -20°C (Shelf Life: 1 year)
    
    * Research Use Only
- Applications
  - Application Description
    
    Dilution: dilute 100 mg of stearyl phosphoramidite in 2.12 mL of anhydrous acetonitrile/dichloromethane 1:3 (v/v) to obtain 0.1 M solution
    Oxidation: standard protocol
    Coupling: 3 minute coupling time is recommended
    Deprotection: standard protocol

Stearyl Phosphoramidite

We Also Recommend

4-Triazolyl Thymidine CED phosphoramidite

4-Triazolyl deoxy Uridine CED phosphoramidite

Beta-L-Thymidine CED phosphoramidite